The Effects of Herbal Medicine Qi Jia Zhen Wu Decoction in Combination with Corticosteroid on the Apoptosis and the Expression of Fas, FasL in Peripheral Blood Lymphocytes from Patients with Systemic Lupus Erythematosus
- VernacularTitle:芪加真武汤联合糖皮质激素体外对SLE患者外周血淋巴细胞凋亡及Fas、FasL表达的影响
- Author:
Ming CHEN
;
Yu TANG
;
Deyu CHEN
- Publication Type:Journal Article
- Keywords:
Qi Jia Zhen Wu Decoction;
Lupus erythematosus, systemic;
Apoptosis;
Antigens, CD95
- From:
Chinese Journal of Dermatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Qi Jia Zhen Wu Decoction (QJZWD), a traditional Chinese medicinal decoction, in combination with corticosteroid on the apoptosis and expression of Fas, FasL in peripheral blood lymphocytes (PBLs) from patients with systemic lupus erythematosus (SLE). Methods QJZWD containing sera were collected from rabbits. PBLs isolated from 19 SLE patients and 10 healthy controls were incubated with QJZWD containing sera (20%, 10%, 5%), dexamethasone (0.001 mol/L), and QJZWD containing sera (20%, 10%, 5%) in combination with dexamethasone. Apoptosis and expression of Fas and FasL of PBL were detected by flow cytometry. Results ①Apoptosis of PBLs cultured in vitro was significantly higher in SLE patients than that in healthy controls (P 0.05). ②Significantly decreased apoptosis was found in PBLs cultured with herb-containing sera (10%, 20%) compared to that without herb-containing sera (P
