Expression of VEGF Eukaryotic Expression Vector in HaCaT Cells and Its Biological Effects
- VernacularTitle:血管内皮细胞生长因子真核表达载体在HaCaT细胞中的表达
- Author:
Naihui ZHOU
;
Weixin FAN
- Publication Type:Journal Article
- Keywords:
Endothelial growth factors;
Enhanced green fluorescent protein;
Hair follicle;
Cells, cultured
- From:
Chinese Journal of Dermatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of transient transfection of human VEGF165 gene into HaCaT cells and the effects of supernatant from transfected cell culture on pig hair follicles in vitro. Methods PIRES2-EGFP-VEGF165 was transiently transfected into HaCaT cells with lipofectamine?2000. Expression of EGFP( enhanced green fluorescence protein, EGFP) was observed by laser confocal microscopy. VEGF in the supernatant was detected by ELISA. Further,the supernatant was added to pig hair follicles cultured in vitro. The growth and morphologic changes of hair follicles were measured. Results PIRES2-EGFP?VEGF165 was successfully transfected into HaCaT cells, which confirmed by laser confocal microscopy and ELISA. Moreover, the supernatant of HaCaT cells transfected with PIRES2-EGFP-VEGF165 could accelerate the growth of pig hair follicles and prolong their anagen phase. Conclusion PIRES2-EGFP-VEGF165 could be successfully transiently transfected into HaCaT cells, and expressed VEGF could upregulate the growth of pig hair follicles in vitro.
