Effect of promoting cellular cholesterol efflux on the apoptosis of foam cells derived from monocytes
- VernacularTitle:促进细胞内胆固醇流出抑制单核细胞源性泡沫细胞凋亡
- Author:
Pei JIANG
;
Pengke YAN
;
Zhongcheng MO
;
Xuan CAO
;
Chaoke TANG
;
Jiangbo WANG
;
Duanfang LIAO
- Publication Type:Journal Article
- Keywords:
Cholesterol;
Foam cells;
Apoptosis;
Lipoproteins, LDL;
Lipoproteins, HDL
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the effect of promoting cellular cholesterol efflux on the apoptosis of foam cells derived from monocytes. METHODS: RAW264.7 cells were incubated with 50 mg/L ox-LDL as a foam cell mode. The apoptosis rate of RAW264.7 cells was assayed by flow cytometry. Cellular lipid droplet was assayed by oil red staining. The rate of cellular cholesterol efflux was assayed with [~3H] label cholesterol, and the content of cellular cholesterol were assayed by high performance liquid chromatography. RESULTS: After incubation with 50 mg/L ox-LDL for 48 h, the content of cellular cholesterol ester increased from (6.8?3.6) mg/g to (101.7?4.5) mg/g (P
