Effect of calcium signal on zinic finger transcription factor and fetal heart gene of cardiomyocytes
- VernacularTitle:细胞内钙信号对锌指转录因子及胚心标志基因的调控
- Author:
Yongjian YANG
;
Xin ZHANG
;
Xingwen ZHOU
- Publication Type:Journal Article
- Keywords:
Calcium;
Myocardium;
Calcineurin;
Mitogen-activated protein kinases
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of intracellular calcium free calcium ([Ca~(2+)]i) on zinic finger transcription factor (GATA_4) and fetal heart gene in cardiomyocytes. METHODS: Cardiomyocytes from fetal rat were cultured primarily. Angiotensin Ⅱ (Ang Ⅱ) and ryanodine (RY) were used to stimulate transmembrane calcium inflow and intracellular calcium release. Fura-2/AM ratio imagine analysis was applied to detect intracellular Ca~(2+) signal. Western blotting were used to measure calcineurin (CaN), nuclear activated T cell factor (NFAT_3), GATA_4 and ?-actin. RT-PCR was applied for observing ?-myosin heavy chain (?-MHC). RESULTS: AngⅡ and RY promoted intracellular free calcium concentration ([Ca~(2+)]i) in cardiomyocytes (P
