Effect of rosiglitazone on myocardial energy substrate utilization in type 2 diabetic rats
- VernacularTitle:罗格列酮对2型糖尿病心肌能量底物代谢的影响
- Author:
Zhongyuan WEN
;
Yongming LIU
;
Teng WANG
;
Pingan ZHANG
;
Xiaolin CHEN
;
Gengshan LI
- Publication Type:Journal Article
- Keywords:
Myocardium;
Insulin resistance;
Rosiglitazone;
Diabetes mellitus
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of rosiglitazone (RSG) to improve insulin sensitivity on myocardial energy substrate utilization as well as the cardiac function in a rat model of type 2 diabetes mellitus. METHODS: Sprague-Dawley rats were conducted into three groups: chow-fed rats were fed with normal chow (12% of calories as fat); fat-fed/STZ rats were fed with high-fat diet (40% of calories as fat) for 4 weeks and then injected with streptozotocin 35 mg/kg intraperitoneal; fat-fed/STZ/RSG rats were fat-fed/STZ rats treated with rosiglitazone (3 mg?kg~(-1)?d~(-1)) for 2 weeks. A cannula connected to a passive transducer was inserted the heart for the measurement of the cardiac function including heart rate (HR), left ventricular end-diastolic pressure (EDP) and ?dp/dt_(max). Then the isolated hearts were mounted onto a Langendorff perfusion apparatus to perfuse with Krebs-Henseleit buffer in the presence of 5 mmol/L glucose and 0.4 mmol/L [~3H] labelled palmitate. Glucose uptake and [~3H_2O] collection were measured to evaluate the rate of carbohydrate and fatty acid oxidation. RESULTS: Compared with the chow-fed rats, fat-fed/STZ rats had a significantly depression of glucose uptake in the hearts [(54.7?6.2 vs 69.0?5.7) ?mol?g~(-1) dry weight, P
