Experimental study on inducing bone mesenchymal stem cells to differentiate into cardiomyocytes in vitro
- VernacularTitle:骨髓间质干细胞体外诱导分化为心肌样细胞的实验研究
- Author:
Yulin WEI
;
Wei WU
;
Jingfeng WANG
;
Yuru FU
;
Jing WEI
;
Yijun DENG
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Mesenchymal stem cells;
Azacitidine;
Cardiomyocytes
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the differentiation of rat bone mesenchymal stem cells (MSCs) into cardiomyocytes in vitro. METHODS: MSCs were isolated and purified from the bone marrow of rats by density gradient centrifugation and adhering to the plastic culture. The third passage MSCs were treated by 5-azacytidine (5-aza). The induced cells were evaluated by immunocytochemistry staining and RT-PCR analysis. RESULTS: After being induced by 5-aza, some MSCs became bigger and longer. The connection of the cells were formed on day 14.The direction of the cells arraying was similar gradually. The induced cells were stained positively for desmin, ?-actin and troponin I. RT-PCR showed that these cells expressed ? myosin heavy chain. CONCLUSION: 5-aza can induce MSCs to differentiate into cardiomyocytes in vitro.
