Effects of promoter region 5′CpG island demethylation on biological phenotype in human colorectal cancer cells
- VernacularTitle:启动子区5′CpG岛去甲基化对人结肠癌细胞生物学表型的影响
- Author:
Xiaoming FANG
;
Shu ZHENG
;
Gongxing CHEN
;
Lifeng SUN
;
Qinghua L
- Publication Type:Journal Article
- Keywords:
Methylation;
Colorectal neoplasms;
Apoptosis;
Cell cycle;
Deoxycytidine
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the relationship between methylation status of promoter region 5′CpG island and the biological phenotype in human colorectal cancer RKO cell lines. METHODS: RKO cells were treated with selective DNA methyltransferase (DNMTs) inhibitor, 5-Aza-2′-deoxycytidine (5-Aza-CdR), for 72 h. Methylation-specific PCR (MSP), T-A clone and DNA sequence analysis were used to detect 5′CpG island methylation status of p16/CDKN2 tumor suppresor gene. Cell growth, cell cycle arrest and apoptosis were analyzed by MTT, flow cytometry (FCM), fluorescent dye staining and transmission electron microscope. RESULTS: DNMTs inhibitor (5-Aza-CdR) effectively reversed the hypermethylation status of 5′CpG island. The effects of 5-Aza-CdR on cell growth inhibition (P
