The Effect of Intraocular Hypothermia on RPE Cell Proliferation.
- Author:
Sung Chul LEE
1
;
Oh Woong KWON
Author Information
1. Department of Ophthalmology, College of Medicine, Yonsei University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Hypothermia;
Proliferative vitreoretinopathy;
Vitrectomy
- MeSH:
Cell Proliferation*;
Epithelial Cells;
Humans;
Hypothermia*;
Inflammation;
Rabbits;
Retinal Detachment;
Retinaldehyde;
Traction;
Vitrectomy;
Vitreoretinopathy, Proliferative
- From:Journal of the Korean Ophthalmological Society
1995;36(9):1498-1502
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effects of intraocular hypothermia on experimental proliferative vitreoretinopathy in rabbits were studied. Vitrectomy was performed for 30 minutes with irrigating balanced salt solution in rabbit eyes, and the cultured retinal pigment epithelial cells were injected into the vitreous cavity. The irrigating balanced salt solution was used at room temperature in control eyes, and was cooled to 6 degrees C in experimental eyes. Experimental eyes showed less postoperative inflammation and demonstrated less occurrence of tractional retinal detachment compared with the control eyes. In vitro, the growth of cultured human retinal pigment epithelial ceDs was studied in the conditions of different temperatures. Significant suppression of cell growth was noted at 43 degrees C and also at 4 degrees C. The cooled irrigating solution may be used during vitrectomy procedure to decrease the occurrence of proliferative vitreoretinopathy.
