Effect of hTERT gene antisense oligodeoxynucleotide on chemotherapeutic drugs-induced apoptosis in CEM cells
- VernacularTitle:hTERT基因反义核酸对化疗药物诱导CEM细胞凋亡的影响
- Author:
Wenyu LI
;
Huan ZHANG
;
Dongmei HE
- Publication Type:Journal Article
- Keywords:
Genes, hTERT;
Oligonucleotides, antisense;
Telomerase;
Cisplatin;
CEM cells;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of human telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide (ASODN) on chemotherapeutic durges-induced apoptosis in CEM cells. METHODS: Cell viability was determined by trypan blue dye exclusion assay. Apoptosis was detected by morphological observation and flow cytomertric cell cycle analysis. RESULTS: The survival rates of CEM cells cultured with daunorubicin, vincristin and (etoposide) respectively were similar with that cultured with those chemotherapic durgs plus hTERT ASODN. The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum (DDP) added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively. CONCLUSION: hTERT ASODN enhances DDP-induced apoptosis of CEM cells.
