Selection and analysis of targeted cell-binding mimicry peptides in endometrial adenocarcinoma cell line through random peptide phage display
- VernacularTitle:子宫内膜腺癌细胞系特异性结合短肽筛选及分析
- Author:
Lin LU
;
Xueyun ZHONG
;
Zineng WANG
;
Yanling YAN
;
Bin DU
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Cell line;
Phage display
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To isolate targeted the peptides that binding and internalizing into endometrial carcinoma cell line EAC. METHODS: The tumor cells were screened five rounds of whole cell screen through the Ph.D.-12 phage display library. Analysis of the displayed peptides specific binding efficiency to the tumor cells was proceeded. The DNA of phages was extracted, sequenced and translated to the sequences of amino acid and analysis using computer software. RESULTS: After five biopannings, the isolated phages showed high specificity and strong affinity for their cognate cell types relative to different cell lines. Through sequencing, the sequences of displayed peptides were obtained. CONCLUSIONS: Whole cell screening against EAC cells through random phage peptide library can obtain phage peptides with a highly tissue specific binding and internalizing ability. The peptides do provide a basis for tumor's targeted delivery as therapy vector.
