Correlative analysis between renal TGF-?1 expression and cell apoptosis in a rat model of cyclosporine-induced chronic nephrotoxicity, and effect of tea polyphenols on TGF-?1 expression and apoptosis
- VernacularTitle:环孢素A慢性肾毒性时肾转化生长因子-?1表达和细胞凋亡间的相关性及茶多酚的影响
- Author:
Feifei MAO
;
Jue WANG
;
Xiaofeng YU
;
Youfa ZHU
;
Haiyang XIE
;
Shaohua SHI
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Tea polyphenols;
Kidney diseases;
Transforming growth factor beta;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the correlation between renal transforming growth factor-?1 (TGF-?1) expression and cellular apoptosis, and effect of tea polyphenols (TP) on TGF-?1 expression and apoptosis. METHODS: Four group animals in a rat model of CsA-induced chronic nephrotoxicity were respectively treated by vehicle (olive oil, 0.1 mL?kg~(-1)?d~(-1), sc), TP (80 mg?kg~(-1)?d~(-1), ig), CsA (15 mg?kg~(-1)?d~(-1), sc) and TP (80 mg?kg~(-1)?d~(-1), ig) plus CsA (15 mg?kg~(-1)?d~(-1), sc). At the end of 28th day of treatment, renal creatinine clearance and tissue pathology were analyzed. The TGF-?1 expression was detected by immunohistochemistry and Western blotting. TUNEL assay, apoptosis-related enzymatic activity caspase-3 were also detected. RESULTS: Compared to CsA-treated rats, the animals treated with CsA plus TP showed a significant increase in the renal creatinine clearance (0.12?0.03 vs 0.22?0.02,P
