Construction of human immunoglobulin combinatorial library containing D-Dimer on the surface of phage
- VernacularTitle:抗D二聚体的人源性噬菌体抗体库的构建
- Author:
Zhaowei TIAN
;
Xiaoyi YANG
;
Wenyan WU
;
Shaohua HUANG
;
Minsheng CHEN
- Publication Type:Journal Article
- Keywords:
Bacteriophages;
Immunoglobulin combinatorial library
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To construct a human recombinant immunoglobulin library containing D-Dimer by using phage surface display technology. METHODS: Human immunoglobulin heavy chain and light chain genes were amplified respectively by RT-PCR from different human lymphocytes using family specific primers and signal sequences of immunoglobulin as half-nested PCR primers. The heavy chain and light chain PCR products were cloned into phagemid vector pComb3H and the human immunoglobulin recombination library was generated with helper phage VCSM13. RESULTS: A human combinatorial antibody library consisting of 2.8?10~8 in dependent clones was generated with a titer of 4.1?10~(17)PFU/L. The recombinant frequency of Fab genes was 46%. CONCLUSION: A human combinatorial antibody library was generated. It will be beneficial for selecting Fab antibodies of D dimer from the library.
