Effect of rhIL-10 on IL-6 and TNF-? levels in serum and liver of lipopolysaccharide-challenged mice
- VernacularTitle:rhIL-10对脂多糖攻击小鼠血及肝IL-6和TNF-?含量的影响
- Author:
Hong YANG
;
Shaohong LUO
;
Hanfang MEI
;
Chunmei LI
- Publication Type:Journal Article
- Keywords:
Interleukin-10;
Interleukin-6;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of recombinant human interleukin-10 (rhIL-10) on IL-6 and TNF-? levels in serum and liver of mice exposed to lipopolysaccharide (LPS). METHODS: rhIL-10 was prepared by using genetic engineering technology. Mice were intraperitoneally with 500 ?g of LPS, and then were treated intravenously with various dosages of rhIL-10. The levels of IL-6 and TNF-? in hepatic tissue and serum were determined by ELISA at 12 h, 24 h, 48 h and 72 h post rhIL-10 treatment. RESULTS: rhIL-10 markedly inhibited the increase in IL-6 and TNF-? levels in hepatic tissue and serum at 12 h after rhIL-10 treatment in LPS-challenged mice, and the inhibition effect was significant at 24-48 h after rhIL-10 treatment (P
