Expression of TCR V? subfamily in patients with idiopathic thrombocytopenic purpura
- VernacularTitle:原发性血小板减少性紫癜患者T细胞受体V?亚家族基因的表达特点
- Author:
Xueli ZHANG
;
Yangqiu LI
;
Jun ZHONG
;
Shaohua CHEN
;
Lijian YANG
- Publication Type:Journal Article
- Keywords:
Receptor, antigen, T-cells;
Genes;
Purpura, thrombocytopenic, idiopathic
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of TCR V? subfamily in T cells in patients with idiopathic thrombocytopenic purpura (ITP). METHODS: The TCR V? 24 subfamily genes were amplified in peripheral blood mononuclear cells from 5 cases with ITP using RT-PCR, to observe the usage of TCR V? repertoire. 10 normal individuals served as controls. RESULTS: Only 4-11 V? subfamily in T cells were identified in ITP cases. The frequent expressions of V? subfamilies were V?2 (100%), V?3 (100%), V?19 (80%) and V?21 (80%), while the expression of V?4, V?6-12, V?17, V?20 and V?24 were not detected. All 24 V? subfamilies were detected in samples from normal individuals. CONCLUSION: The restricted expression of TCR V? subfamilies is one of the T-cell immune features in patients with ITP, indicating that it may be related to the disorder of cellular immune function in ITP.
