Influence of laminin on the expression of bcl-2 in rabbit cornea endothelial cells
- VernacularTitle:层粘连蛋白对兔角膜内皮细胞bcl-2表达的影响
- Author:
Xiaoqian ZHU
;
Jing WU
;
Jintang XU
;
Songbin ZHAO
- Publication Type:Journal Article
- Keywords:
Laminin;
Genes, bcl-2;
Gene expression;
Rabbit;
Cornea;
Endothelial cells
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the influence of laminin (LN) on the expression of bcl-2 in rabbit cornea endothelial cells. METHODS: Cultured rabbit corneal endothelial cells were divided into three groups: normal control group, LN group and negative control group. Immunohistochemical technique and ELISA were used to measure the staining and A value to assess the levels of bcl-2 expression. RESULTS: Bcl-2 expression score showed that LN group had a strong positive expression. Control group only showed a weak expression. Negative control group showed a negative expression by immunohistochemical staining. The mean A value of each group were 1.21?0.18 (LN group), 1.05?0.14 (normal control group) and 0.04?0.01 (negative control group). CONCLUSION: LN promotes bcl-2 gene expression in rabbit cornea endothelial cells, and protects the cells from apoptosis.
