Preparation and Evaluation of BMP_2 Carrying Gel Microspheres by Dextran-glycidyl Methacrylate
- VernacularTitle:右旋糖酐-甲基丙烯酸缩水甘油酯载BMP_2凝胶微球的制备与评价
- Author:
Faming CHEN
;
Zhifen WU
;
Guofang WANG
;
Yan DU
;
Yan JIN
- Publication Type:Journal Article
- Keywords:
Dextran;
Glycidyl methacrylate;
Bone morphogenetic protein-2;
Gel microspheres;
Controlled release;
Or-thogonal test;
Preparation technology
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJRCTIVE:To study the feasibility and the preparation of bone morphogenetic protein-2(BMP 2 )carrying gel microspheres by dextran-glycidyl methacrylate(DEX-GMA)and to make an initial study on the drug-loading and drug releasing function of gel.METHODS:The orthogonal test was conducted with the reaction temperature,the addition of DEX-GMA and Span-80,the stirring speed and so on as investigation factors,the best preparation technics of BMP 2 -DEX-GMA gel microspheres was optimized and the finished products of which were given an initial determination.RE-SULTS:The BMP 2 -DEX-GMA gel microspheres preparation could be made by suspension polymerization,the optimized preparation technics including the reaction temperature was30℃,the addition of DEX-GMA and Span-80were0.6%and0.06%respectively,the stirring speed was300r/min,the BMP 2 -DEX-GMA gel microspheres from the above formulation take good shapes with the particle diameter at20?m～50?m,the envelopment ratio was(78.52?4.34)%,the quantity of drug-loading was(10.68?1.34)%,and the swelling ratio was85.9%,which has a good stability and redispersibility.CON-CLUSION:The preparation technics of gel microspheres drug-loading system is simple and the loading dosage is high,which can be used as a bioactive drugs carrier.
