Determination of Berberine Hydrochloride in Rabbit Plasma by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定兔血浆中盐酸小檗碱的浓度
- Author:
Yue WEN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Berberine hydrochloride;
Rabbit;
Blood concentration
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish RP-HPLC method for the determination of berberine hydrochloride in rabbit plasma.METHODS:RP-HPLC internal standard method was adopted in which Dikma diamonsil TM C 18 was used as chro-matographic column,the mobile phase was composed of acetonitrile-0.1mol/L potassium dihydrogen phosphate solution-sodium dodecyl sulphate(50∶50∶0.06)with flow rate at1ml/min,and the detection wavelength was345nm,the column temperature was40℃with paradimethylaminobenzal dehyde taken as the internal standard.RESULTS:Good linear relation-ship was achieved when the detection concentration of berberine hydrochloride was within the range of20.5～656ng/ml(r=0.9999),the average recovery rate was99.14%,and the mean RSD was3.78%.CONCLUSION:The method has high sen-sitivity and strong specificity,which can be used for the study of pharmacokinetic of berberine hydrochloride.
