Determination of Principal Components in Levofloxacin Hydrochloride Ointment by HPLC
- VernacularTitle:高效液相色谱法测定盐酸左氧氟沙星软膏中主药的含量
- Author:
Xianhong ZOU
;
Wei PENG
;
Yongjun XIAO
;
Meirong ZHU
- Publication Type:Journal Article
- Keywords:
HPLC;
Levofloxacin hydrochloride;
Ointment;
Content determination
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of levofloxacin hydrochloride in the levoflo_ xacin hydrochloride ointment.METHODS:The chromatographic column was C 18 ,the mobile phase was consisted of0.05mol/L citric acid-acetonitrile(85∶15)with detection wavelength at293nm and flow speed at1.0ml/min,the column temperature was40℃and the sample size was10?l.RESULTS:Good linear relation was achieved when the levofloxacin hydrochloride concen-tration was at the range of3.88～37.88ug/ml(r=0.9998);The average recovery was99.4%(n=9).CONCLUSION:This determination method is accurate,reliable and it can act as an effective quality control for levofloxacin hydrochloride ointment.
