Detection of Estrogen Receptor in Breast Cancer by Flow Cytometry
- VernacularTitle:运用流式细胞术对乳腺癌激素受体检测的研究
- Author:
Huiling YUAN
;
Yanqun WU
;
Lin ZHANG
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Estrogen receptor;
Progestogen receptor;
S phase fraction;
Ploid;
Flow cytometry
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect estrogen receptor and DNA index in breast cancer by flow cytometry. Methods 32 cases of fresh specimens of breast cancer resected by operation were used in this study. Single cells suspension was prepared from those specimens, and estrogen receptor expression was analyzed by flow cytometry. At the same time, the status of ploid and S phase cell ratio were determined. Results Flow cytometry analysis could measure the expression level of estrogen receptor in the fresh breast cancer specimens, which was more sensitive and needed less volume of tumor tissue compared with immuohistochemical method. The information on the status of ploid and S phase cell ratio were simultaneously obtained. Conclusion The detection of estrogen receptor in breast cancers by flow cytometry was more helpful for evaluating prognosis and selecting treatment.
