A study on the biological characters of SGC7901 gastric cancer cell-dendritic cell fusion vaccine
- VernacularTitle:SGC7901胃癌细胞-树突状细胞融合疫苗生物学特性的研究
- Author:
Kun ZHANG
;
Pengfen GAO
;
Peiwu YU
;
Yun RAO
;
Lixin ZHAO
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Cancer vaccine;
Dendritic cell
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide experimental basis for the study of SGC7901 gastric cancer cell-dendritic cell fusion vaccine. Methods The fusion of SGC7901 gastric cancer cells and dendritic cells was induced by PEG. We obtained pure fusion cells by selective culture with HAT/HT culture systems. The growth curve characters, clone abilities, abilities to irritate T lymphocytes and the ability to grow were detected. Results The fusion cells had weaker proliferative abilities and clone abilities with increased abilities to irritate T lymphocytes. The CPM value of T lymphocytes were 15083?231、 9608?83、 4214?135、 3020?28 respectively when they were co-cultured with the fusion cells on different proportions. Conclusion SGC7901 gastric cancer cell-dendritic cell fusion cells keep strong abilities to irritate T lymphocytes while inable to grow into new planted tumors in immunodeficiency animals.
