An experimental study on vitamin K3 induced HEPG2 cell line apoptosis
- VernacularTitle:维生素K_3促肝癌细胞凋亡的实验研究
- Author:
Tong WANG
;
Chen WANG
;
Xiong ZHOU
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Vitamin K;
Apoptosis;
Caspase-3;
Gene, Bcl-2
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of vitamin K3 (VitK3) on the apoptosis of human hepatocellular carcinoma cell line of HEPG2 and its relationship with Caspase-3 and Bcl-2 gene. Method With the treatment of VitK3, time and dosage effect was detected by MTT colorimetry. Appoptosis was observed by morphology, flow cytometry and DNA agarose gelelectrophoresis.Caspase-3 and Bcl-2 mRNA expression was assessed by RT-PCR. ResultVitK3 significantly inhibits HEPG2 cell proliferation and induce apoptosis.Caspase-3 mRNA expression was upregulated. ConclusionVitK3 induces HEPG2 cell apoptosis by way of Caspase-3 gene expression.
