HPLC Determination of Ketoconazole and Desamethasone Acetate in Ketoconazole Spraying Film Simultaneously
- VernacularTitle:高效液相色谱法同时测定酮康唑喷膜中2组分的含量
- Author:
Xuemei YI
;
Wenji WANG
;
Chun ZHANG
;
Jianhua WU
;
Hai WEN
- Publication Type:Journal Article
- Keywords:
HPLC;
Ketoconazole spraying film;
Ketoconazole;
Desamethasone acetate;
Content determination
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an approach to determination of ketoconazole and desamethasone acetate in novel ketoconazole spraying film.METHODS:Chromatography was performed using a Waters Symmetry C 18 .The mobile phase consisted of methyl-monopotassium phosphate(4∶1).Detection wave was at239nm.The flowing rate was0.6ml/min.RESUL_ TS:Excellent linear correlations were showed when the concentrations of ketoconazole and desamethasone acetate were in the ranges of0.2～2mg/ml and0.02～0.2mg/ml respectively.The mean recoveries of ketoconazole and desamethasone acetate were98.41%(RSD=2.29%,n=6)and97.84%(RSD=2.60%,n=6)respectively.CONCLUSION:The established approach is precise,reliable and therefore applicable to the quality control of novel ketoconazole spraying film.
