Detection of Serum Chemokines and Their Receptors on Peripheral Blood CD4~+ T Lymphocytes in Patients with Atopic Dermatitis
- VernacularTitle:特应性皮炎患者趋化因子及其受体的研究
- Author:
Qifeng QIAN
;
Lihang LIN
;
Mingxia ZHANG
;
Qiwen LIU
- Publication Type:Journal Article
- Keywords:
Dermatitis,atopic;
Chemotactic factors;
Receptors,chem okine
- From:
Chinese Journal of Dermatology
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of serum chemokines and their receptors in the pathogenesis of atopic dermatitis(AD).Methods Serum levels of interferon ?-inducible protein-10(IP-10),stromal cell-derived factor-1? (SDF-1?),eotaxin,thymus and activation-regulated chemokine(TARC) as well as macrophage-derived chemokine (MDC) were detected by enzyme-linked immunosor bent assay(ELISA) in 39 patients with AD and 39 normal controls.Meanwhile,chem okine receptors CXCR3,CXCR4,CCR3,CCR4 and CCR5 on peripheral blood CD4+T-lymp hocytes were analyzed by flow cytometry with a two-color immunofluorescent sta ining in 39 patients with AD.Results Serum levels of SDF-1?,TARC and MDC we re significantly higher(P 0.05) between the patients[(123.6 ? 110.4) pg/mL a nd (68.7 ? 26.2) pg/mL,respectively]and controls [(100.7 ? 73.7) pg/mL and(66.8 ? 20.5) pg/mL,respectively].The expression of CXCR3,CCR3,CCR4 and CCR5 o n CD4+T cells was increased (P
