Detection of alpha-thalassemia gene by coelocentesis in early prenatal diagnosis
- VernacularTitle:运用胚外体腔液产前诊断?地中海贫血的临床研究
- Author:
Fengzhen ZHOU
;
Jiani LI
;
Xiufeng YE
- Publication Type:Journal Article
- Keywords:
alpha-Thalassemia;
Prenatal diagnosis;
Polymerase chain reaction
- From:
Chinese Journal of Perinatal Medicine
1998;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the safety and accuracy of diagnosing alpha-thalassemia with extraembryonic coelomic cells. Methods Coelocentesis was performed before artificial abortion to collect extraembryonic coelmic fluid in 40 women with singleton pregnancy during 6-10 gestational weeks. The villi was gathered after suction. PCR technique was used to amplify alpha-thalassemia gene in both extraembryonic coelomic cells and villi and concordant rate of two different kinds of samples were compared. Results The genotypes of alpha-thalassemia were successfully amplified in 37 cases of coelomic cells and all were concordant with the results of villi. Conclusion Extraembryonic coelomic cells can be used in early prenatal diagnosis of alpha-thalassemia by PCR technique and is practicable.
