Study on active oxygen metabolism of neutrophils in neonates
- VernacularTitle:新生儿中性粒细胞活性氧代谢水平的研究
- Author:
Jun BU
;
Jianhua SUN
;
Ping HUANG
- Publication Type:Journal Article
- Keywords:
Neutrophils;
Infant, newborn;
Escherichia coli;
Reactive oxygen species
- From:
Chinese Journal of Perinatal Medicine
1998;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the causes of reduced function of neutrophils in neonates by measuring superoxide, one of the reactive oxygen species, produced by neutrophils following live bacterial phagocytosis in the whole blood. Methods Twenty-three cord blood samples were collected from normal full-term newborns and cultured with live Staphylococcus aureus and Escherichia coli. Blood cells were stained with Hydroethidine, an indicator of superoxide. The intensity of the superoxide of neutrophils were analyzed by flow cytometry. Fifteen adult blood samples were collected as control. Plasma replacement was used to study the function of body fluid in active oxygen metabolism of neutrophils. Results Most full-term neonatal neutrophils (PMNs) could produce superoxide anion following bacterial phagocytosis in the blood as that in adults. The level of superoxide production of term neonatal neutrophils, after stimulation of S. aureus, was as high as the adult′s but was significantly lower after stimulation by E. coli (bacteria cell ratio 40∶1, MFI 347?70 vs 461?55, P
