Clinical study of different methods on early diagnosis and monitoring of neonatal congenital cytomegalovirus infection
- VernacularTitle:不同实验方法在诊断和监测新生儿先天性巨细胞病毒感染中的临床研究
- Author:
Yijuan LI
;
Yu ZENG
;
Siqi ZHUANG
- Publication Type:Journal Article
- Keywords:
Cytomegalovirus infections;
Infant, newborn;
DNA,viral;
Polymerase chain reaction;
Immunoglobulin M;
Antigens,viral
- From:
Chinese Journal of Perinatal Medicine
1998;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility and utility of three different assays on early diagnosis and monitoring of neonatal congenital cytomegalovirus infection. Methods Ninety-eight neonates whose mother was CMV-IgM positive during pregnancy were examined on the 14th days after birth for CMV antigen in blood and PCR-CMV-DNA in the saliva. Three different methods were applied including CMV antigenemia assay, PCR for CMV-DNA and ELISA for serum CMV-IgM. Neonates were followed up for six months. Results (1) Forty-eight of the 98 neonates were diagnosed as congenital CMV infection including 7 symptomatic infection and 41 asymptomatic. None of the 98 subjects was CMV-IgM positive. Among the 7 symptomatic cases, the positive rates of CMV antigen and PCR-CMV-DNA were 100%(7), 71.4%(5), and 70.7%(29/41), 46.3%(19/41) in the asymptomatic group, respectively. The sensitivity of CMV antigenemia assay and PCR was 75.0% and 54.2%, respectively. The CMV antigenemia index of the symptomatic cases was significantly higher than that of asymptomatic ones [(16-52)/50 000 vs (3-31)/50 000 white blood cells, P
