Caveolin-1 expression is downregulated by shear stress and TNF-? in human aortic endothelial cells
- VernacularTitle:切应力和TNF-?调节人血管内皮细胞质膜微囊蛋白的表达
- Author:
Qing XIANG
;
Xi DONG
;
Mei XU
;
Ruijuan SUN
- Publication Type:Journal Article
- Keywords:
Endothelial cells;
Caveolin 1;
Shear stress;
TNF;
Atherosclerosis
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe effects of shear stress and TNF-? on caveolin-1 expression. METHODS: Cultured human aortic endothelial cells (HAECs) of passage 3-5 were used in the experiment. Cells were exposed to a laminar flow (shear stress 1.0 Pa) by using a parallel rectangular flow chamber for different time. Caveolin-1 mRNA and protein expression were measured by RT-PCR and Western blot, respectively. Caveolin-1 expression of the cells stimulated by TNF-? were also studied to elucidate the influence of this inflammatory factor. RESULTS: After 24 h of exposure to 1.0 Pa shear stress, both of caveolin-1 protein and mRNA expression decreased in HAECs, especially caveolin-1 mRNA expression (P
