Experimental study on induction of atherosclerotic plaque instability in rabbits after transfer of wild-type p53 gene
- VernacularTitle:转染野生型p53基因诱发家兔动脉粥样硬化斑块不稳定性的实验研究
- Author:
Wenqiang CHEN
;
Yun ZHANG
;
Mei ZHANG
;
Xiaoping JI
;
Chen LIN
;
Yongfeng ZHU
;
Xuedong SANG
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Genes, p53;
Transfection;
Arteriosclerosis
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the apoptotic role of wild-type p53 in induction of plaque instability in atherosclerotic rabbits. METHODS: Fifty-four New Zealand White rabbits underwent balloon-induced abdominal aortic wall injury and then were fed on a diet of 1% cholesterol. At the end of the eighth week, the rabbits were randomly divided into two groups: group A and group B. Recombinant adenovirus carrying p53 and ?-galactosidase (LacZ) genes were injected in group A and B, respectively. Two weeks later, 10 rabbits each in group A and B was killed and the remaining rabbits all underwent pharmacological triggering with injection of Chinese Russell's viper venom and histamine. RESULTS: Compared with group B, p53 gene over-expression in group A resulted in a marked increase in number of positive apoptotic cells (2.5%?0.8% vs 1.0%?0.3%, P
