Effect of nuclear factor-?B on the anti-apoptosis induced by focal ischemia preconditioning in Wistar rats
- VernacularTitle:NF?B在大鼠局灶性脑缺血预处理抗细胞凋亡中作用的研究
- Author:
Yanqiu DENG
;
Gang ZHAO
;
Zheng ZHAO
;
Jianzhi WANG
- Publication Type:Journal Article
- Keywords:
Ischemic preconditioning;
Brain ischemia;
Apoptosis;
NF-kappa B;
Superoxide dismutase;
Rats
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of nuclear factor-?B (NF-?B) on the anti-apoptosis induced by brain ischemia preconditioning (IP). METHODS: Temporary middle cerebral artery occlusion for 20 min followed three days reperfusion before 6 hours middle cerebral artery occlusion (MCAO) trancranially was used as preconditioning in Wistar rats. The protective role was evaluated by analyzing the infarct volume. The status of neuronal apoptosis was observed by TUNEL. The expression of NF?B p65 protein, the assay of SOD activity and MDA concentration were analyzed by using the methods of immunohistochemistry and cytochemistry. RESULTS: Compared to the control group, 20 min ischemic preconditioning, which did not produce neuronal damage obviously, reduced the infarct volume significantly after MCAO 6 h and obviously decreased the number of neural cell apoptosis in penumbra (P
