Establishment and biological characteristics of a new human endometrial carcinoma
- VernacularTitle:人子宫内膜癌细胞系的建立及其生物学特性研究
- Author:
Lin LU
;
Bin DU
;
Jihong LIU
;
Junsheng ZHENG
;
Zineng WANG
;
Xueyun ZHONG
- Publication Type:Journal Article
- Keywords:
Uterine neoplasms;
Cell line;
Tumor cells, cultured
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a new human endometial carcinoma cell line for basic and clinical study. METHODS: The minced tumor tissues were inoculated subcutaneously into the back of nude mice. After forming solid tumor, the mice were killed and the transplant was taken out for primery culture. RESULTS: The established cell line was maintained by serial passages, named EAC. EAC cell line grew rapidly,steadily, and it had similar microscopic morphology to adenocarcinoma cell. Chromosome analysis reveal EAC chromosome counts ranging in hyperdiploid. The expression of ER, PR, P53, MDM2 were negative by SP immunohistochemistry. Tumorigenicity of EAC in nude mice was 100%. CONCLUSION: A new human endometrial carcinoma, EAC, is established successfully, which is helpful for the basic and clinical study of endometrial carcinoma.
