Study on Extraction Technique of Whitmania Pigra Whiman
- VernacularTitle:宽体金线蛭提取工艺研究
- Author:
Lan YU
- Publication Type:Journal Article
- Keywords:
Hirudo extract;
Extraction technique;
Biuret colorimetry;
Content determination;
Activated partial throm?boplastin time
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To select an optimal extraction technique of Whitmania pigra whiman.METHODS:The af?fecting factors such as variety of solvent,temperature,time,the amount of solvent and the frequency of the extraction were studied on the basis of protein content in the hirudo extract and activated partial thromboplastin time(APTT).RESULTS:The optimum extraction result was obtained in4%NaCl solvent of10-fold as much as the medicine with3times of extraction at60℃for6h((3h+2h+1h).CONCLUSION:The present technique is simple,scientific and practicable.
