Determination of Gallic Acid in Viviparous Bistort Rhizome by HPLC
- VernacularTitle:高效液相色谱法测定蝎子七中没食子酸的含量
- Author:
Hanping WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Viviparous bistort rhizome;
Gallic acid;
Content determination
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of gallic acid in viviparous bistort rhizome by HPLC.METHODS:The chromatographic column was Nova-Pak C 18 and the mobile phase was0.2%methanol methyl cyanide solution-0.1%phos?phoric acid-0.15%triethylamin(3∶50∶47),the flow rate was1.0ml/min and the detection wavelength was271nm.RE?SULTS:It took a good linear relationship when the sample size of gallic acid was within the range of0.028～0.14?g(r=0.9991),the average recovery was99.05%(RSD=4.0%).CONCLUSION:The method is convenient,fast,reproducible,which can be used for the content determination of gallic acid in viviparous bistort rhizome.
