Expression and significance of toll-like receptor 2, 4 in peripheral blood mononuclear cells in patients with systemic inflammatory response syndrome
- VernacularTitle:全身炎症反应综合征患者外周血单个核细胞Toll样受体2、4基因表达及其意义
- Author:
Yang WANG
;
Heshui WU
;
Jing XIONG
;
Jinxiang ZHANG
;
Chunyou WANG
- Publication Type:Journal Article
- Keywords:
Abdomen, acute;
Toll-like receptors;
Gene expression;
Systemic inflammatory response syndrome
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore changes of toll like receptor(TLR) 2, 4 in peripheral blood mononuclear cells (PBMC) in acute abdomen patients with systemic inflammatory response syndrome(SIRS) and their significance. MethodsA clinical study was done on 103 patients in which 65 were with SIRS. The mRNA expression of TLR2, 4 were detected by RT PCR; the expression of TNF ? and IL 6 were observed by ELISA; the correlation between TLR2, 4 mRNA, the level of TNF ? and IL 6,and the clinical course was evaluated. ResultsTLR2 mRNA, TNF ? and IL 6 were upregulated markedly on the first day of hospitalization, then decreased gradually; TLR2mRNA maintained on high level till the 5th day. The expression of TLR2, 4 mRNA was positive correlated with the level of TNF ? and IL 6, and the length of stay. TLR2, 4 mRNA expression increased in patients with multiple organ failure. ConclusionsIn acute abdomen patients with SIRS, the expression of TLR2, 4 of PBMC increased markedly, indicating its important role in the pathogenesis of SIRS.
