Expression of transforming growth factor-?1 and its type Ⅰ receptor in autogenous vein grafts in rats
- VernacularTitle:转化生长因子?1及其Ⅰ型受体基因在大鼠自体移植静脉中的表达
- Author:
Daxin SUN
;
Qiang ZHANG
;
Xiaoou LANG
;
Minghui LIU
;
Zhihong ZONG
- Publication Type:Journal Article
- Keywords:
Tunica intima;
Hyperplasia;
Transforming growth factor beta;
Receptor
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of TGF ?1 and type Ⅰ receptor and their relations with intimal hyperplasia in autogenous vein grafts in rats. Methods Autogenous vein graft model was established in 48 Wistar rats. The vein grafts were harvested on day 3, 7, 14, and 28.Histomorphological methods were used to measure the thickness of intima and wall at different time points. Immunohistochemistry and Western blot were used to detect the protein expression of TGF ?1 and TGF ?RⅠ . RT PCR was used to detect their mRNA level. ResultsThe intimal thickness increased on day 7 compared with controls( P
