Protective effect of estrogen on rat′s retina injured by transient ischemia-reperfusion
- VernacularTitle:雌激素对鼠视网膜缺血再灌注所致视网膜损伤的保护作用
- Author:
Yan DAI
;
Xiaoming CHEN
;
Xiaohu CHEN
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Retinal vessels;
Cell death;
Estrogens/physiology;
Disease models, animal
- From:
Chinese Journal of Ocular Fundus Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effect of estrogen on survival of retinal ganglion cells (RGCs) after transient retinal ischemia-reperfusion (RIR) in rats. Methods RIR was induced in 60 ovariectomized adult rats (OVX) by increasing intraocular pressure via an intracameral catheter. All of the rats were divided into two groups randomly: in experimental group, the rats underwent a subcutaneous injection with 17?-estrodiol(100 ?g/kg) 2 hours before retinal ischemia; and in the control group, saline water was injected correspondingly. The number of RGCs and the thickness of the inner retinal layers were mesured by HE staining method before and 12, 24, 48, and 72 hours after reperfusion. TdT-mediated biotin-dUTP nick end labelling (TUNEL) staining technique was used to examine the apoptosis of RGCs. Results Twenty-four and 48 hours after reperfusion, the number of apoptotic cells in experimental group was obvious lower than that in the control group( P
