The Induction of Cytokine-induced Killer and its Cytotoxic Effect on Hepatic Carcinoma Cells
- VernacularTitle:CIK细胞的诱导及其对肝癌细胞毒作用的体外研究
- Author:
Liyi HU
;
Youshun ZHANG
;
Xin ZHOU
- Publication Type:Journal Article
- Keywords:
CIK;
Hepatic carcinoma cell;
Phenotype;
Cytotoxity
- From:
Journal of Chinese Physician
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare cytokine-induced killer(CIK) in vitro, and study the biological activities of CIK. Methods Lymphocytes were isolated from peripheral blood and umbilical blood, and induced by cytokine.The cell surface markers CD_3 and CD_~56 of CIK were analyzed by FACS. The proliferation of CIK was tested using 3H -TdR release method, and the cytotoxic effect of CIK on hepatic carcinoma cells was measured by MTT. Results CIK cells quickly proliferated from the second week, and expanded more than 60-fold at the 31th day after induction. The CD_3+ and CD_~56 + cells expanded more than 800 folds. The cytotoxic effect of CIK on hepatic carcinoma cells was obviously stronger than that of LAK cells, and CIK had not obvious cytotoxic effect on fetal liver cells. Conclusion CIK was a highly efficient cytotoxic cells against tumors, and had clinical application potentials.
