The Effect of IL-4, 12 on the Airway Inflammation in Rat Asthma
- VernacularTitle:支气管哮喘气道IL_4和IL_(12)动态变化及对气道炎症反应的影响
- Author:
Liwen OU
;
Ping ZHANG
;
Zuosheng YANG
- Publication Type:Journal Article
- Keywords:
Asthma;
Eosinophil;
IL-4;
IL-12
- From:
Journal of Chinese Physician
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the level change of interleukin-4(IL-4) and IL-12, and their effect on airway inflammation in rat asthma. Methods Rat model of asthma was set up by ovalbumin sensitization. Total cell count and eosinophil count in branchoalveolar lavage fluid(BALF) were performed, and levels of IL-4 and IL-12 in BALF were measured by ELISA. Results After ovalbumin sensitization, airway occurred obvious inflammation. Eosinophil count began increasing at 1 hour after sensitization, obviously increased at 8 hours, reached a maximum at 24 hours, started declining at 96 hours, and decreased to near normal range at 7 to 14 days. The changes of total cell count had the same tendency as eosinophil count changes. The level of IL-4 in BALF elevated at 1h after sensitization, reached a maximun at 8 to 96 hours, and reduced to near normal level at 7 to 14 days. The changes of IL-12 in BALF had the same tendency as IL-4 level changes. Eosinophil count was positively related with IL-4 level(r=0.74,P0.05). There was a close correlation between IL-4/IL-12 ratio and eosinophil count (r=0.85,P
