The Effect of Levo-anlodipine on Myocardial Cells Calcium Overload in Anoxic Condition
- VernacularTitle:左旋氨氯地平对缺氧心肌细胞钙超负荷的作用
- Author:
Kaichao LONG
;
Tong LI
;
Changjiang XIAO
- Publication Type:Journal Article
- Keywords:
Myocardial cell;
Anoxia;
Calcium overload;
Ca~(2+) ATPase;
Levo-anlodipine;
Gene expression
- From:
Journal of Chinese Physician
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of levo-anlodipine on the calcium overload in the anoxic myocardial cells. Methods Myocardial cells were cultured, and in vitro model of anoxic myocardial cells was established. Fura2/am fluorescent probe was used to measure the free calcium concentration of the myocardial cells by spectrofluorometer. The expression of Ca~2+ ATPase of cellular membrane was detected by RT-PCR, and the content of calcium pump in sarcoplasmic reticulum was measured by western blot. Results The levo-anlodipine could decrease the free calcium concentration of the anoxic myocardial cells markedly(359.06?75.00nM), and increase the expression of Ca~2+ ATPase of cellular membrane and the content of calcium pump of sarcoplasmic reticulum in the anoxic myocardial cells. Conclusion Levo-anlodipine could enhance the resistance to calcium overload in anoxic myocardial cells through increasing the expression of Ca~2+ ATPase.
