Determination of Blood Concentration of Pioglitazone Hydrochloride by HPLC
- VernacularTitle:高效液相色谱法测定人血浆中盐酸吡格列酮浓度
- Author:
Jiangping LIAN
;
Peng ZHANG
;
Xiaochuan LI
;
Qiaoru LI
;
Shihu CHEN
;
Jianwu GAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Pioglitazone hydrochloride;
Blood concentration
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method of determining the blood concentration of pioglitazone hydrochloride. METHODS:Chromatographic column was based on Hypersil C l8 (150mm?4.6mm,5?m),the mobile phase consisted of ace-tonitrile-buffer phosphate(40∶60,V/V)with a flow rate at l.0ml/min,the detecting wavelength was229nm.The blood sample was extracted with dichlormethane.RESULTS:The linear concentration range was25～4000ng/ml(r=0.9998,n=8).The lowest detecting concentration was25ng/ml.The extracting recovery rate of high,medium and low concentrations were(73.33?1.22)%,(76.92?6.57)%and(84.50?3.40)%respectively,the method recovery which were(103.26?3.31)%,(97.31?9.07)%and(99.61?6.48)%respectively.The intra-day RSD
