Establishment of High Performance Liquid Phase Fingerprint of Chuanxiong
- VernacularTitle:川芎高效液相指纹图谱的建立
- Author:
Jinchun SONG
- Publication Type:Journal Article
- Keywords:
Chuanxiong;
HPLC;
Fingerprint;
Quality control
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish high performance liquid phase fingerprint for chuanxiong so as to control its quali-ty.METHOD:The chromatographic column was Zorbax SB-C 18 with column temperature at25℃and flow rate at1.0ml/min;the detection wavelength was323nm;the gradient elution was conducted with methanol and0.1%glacial acetic acid water solution.To study the effects of chuanxiong by different extraction methods on fingerprints;the isocratic elution was conducted to investigate the fingerprints of chuanxiong under different detecting wavelength.RESULTS:The ultrasonic method had a good effect in the gradient elution.CONCLUSION:The method is simple,reliable and reproducible,which can be used to es-tablish fingerprint for chuanxiong.
