Inhibition of Malignant Melanoma Cell Growth Through Blocking Expression of VEGF by RNA Interference
- VernacularTitle:血管内皮生长因子-siRNA对恶性黑素瘤细胞生长的抑制作用
- Author:
Yating TU
;
Juan TAO
;
Changzheng HUANG
;
Xingping ZHANG
;
Lixia ZHANG
;
Guanxin SHEN
- Publication Type:Journal Article
- Keywords:
Melanoma;
Endothelial growth factors;
siRNA;
Apoptosis
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To construct a small interfering RNA (siRNA) targeting vascular endothelial growth factor (VEGF) in eukaryotic expression vector, and to assess effects of this vector on proliferation and apoptosis of malignant melanoma cell line A375. Methods pU-VEGF-siRNA plasmid was transfected into A375 cells by electroporation. VEGF mRNA and protein were detected by reverse transcription polymerse chain reaction (RT-PCR) and ELISA, respectively, in A375 cells after gene transfer. Proliferation of A375 cells was assessed by cell counting. Human umbilical vein endothelial cells (ECV-304) were cultured in medium containing supernatants of treated and untreated A375 cells, and their viability was tested by cell counting. Apoptosis of A375 cells was observed by flow cytometry (FCM). Results VEGF mRNA and protein were significantly decreased in the experimental group, compared to those in the controls (P
