Changes of hippocampal neuron microenvironment and contents of amino acid after focal cortical brain ischemia in tree shrews
- VernacularTitle:树皮层局部脑缺血时海马细胞微环境及氨基酸含量动态变化的研究
- Author:
Gang ZHAO
;
Shuqing LI
- Publication Type:Journal Article
- Keywords:
Photochemistry;
Tupaiidae;
Brain ischemia;
Hi ppocampus;
Amino acids
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the changes of hippocampal ne uronal microenvironment and alterations of excitatory amino (EAA) and inhibitory amino acid (IAA) in extracellular fluid (ECF) in different time after t hrombotic cerebral ischemia in tree shrews. METHODS: The model of focal thrombotic cerebral ischemia was ind uced by photochemistry-technology in tree shrews. Hippocampal ECF was collected b y microperfusion. pH, PCO 2, PO 2 and HCO 3- were analyzed by blood gas ana lyzer, and Asp, Glu, Gly and GABA were measured by high-performance liquid chrom atography (HPLC)-PITC technology after occlusion. RESULTS: The contents of Asp, Glu, Gly and GABA in hippocampal E CF increased, and pH, PO 2 and HCO 3- decreased after photochemical induced cerebral ischemia in tree shrews. There were significant differences between ish emic group and sham group (P
