Effects of testosterone on the fibrinolysis activity of HUVEC and its mechanism
- VernacularTitle:睾酮对人血管内皮细胞纤溶活性影响及机制
- Author:
Hong JIN
;
Lu FU
;
Yifang MEI
;
Lijun ZHOU
;
Shaojun LI
- Publication Type:Journal Article
- Keywords:
Testosterone;
Tissue plasminogen activator;
Plasminogen activator inhibitor 1;
Endothelial cells;
Receptors, androgen
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of testosterone with varied concentrations on the fibr inolysis activity of HUVEC and its mechanism. METHODS: Human umbilical vein endothelial cells (HUVEC) were cul tured as recommended. After confluence, the cultures were treated with testoster one(3 ?10 -10, 3?10 -9, 3?10 -8,3?10 -6, 3?10 -5 m ol/L) , and the control confluent cells were cultured in the same medium witho ut steroid. MTT experiment was repeated for 72 hours to investigate each groups' cell proliferation. The tPA and PAI-1 antigen levels were assayed with ELISA K its. Then with HUVEC incubated in androgen receptor antagonist (flutamide) 3 hou rs previously, the experiment was repeated. RESULTS: Testosterone at physiologic or lower concentrations (3 ?10 -10 to 3?10 -8 mol/L ) stimulated the secretion of tPA by HUVEC (P
