The in vitro immune effects of dendritoma formed by mouse hepatocellular carcinoma cells and lymphotactin gene modified dendritic cells
- VernacularTitle:淋巴细胞趋化因子增强肝癌树突状细胞融合瘤苗体外免疫作用
- Author:
Hao ZHANG
;
Shusen ZHENG
;
Guoping JIANG
;
Lihua WU
;
Yunle WAN
;
Haiyang XIE
- Publication Type:Journal Article
- Keywords:
Lymphotactin;
Dendritic cells;
Cell fusion;
L iver neoplasms
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the in vitro anti tumo r immune responses of dendritoma formed by mouse hepatocellular carcinoma cells and lymphotactin gene modified dendritic cells (DCs). METHODS: DCs prepared from mouse bone marrow were genetically mo dified by lymphotactin adenovirus, and fused with H22 cells by polyethylene glyc ol. RT-PCR and ELISA were employed to identify lymphotactin expression at mRNA a nd protein levels. The phenotypes and fusion efficiency were detected by FACS. T he stimulatory capacity of DC to T cells was detected by mixed leukocyte reactio n. The cytotoxicity activity against H22 cells was assayed by LDH method. RESULTS: Lymphotactin effectively expressed by DCLptn/H22 hybrid oma. DCLptn/H22 cells induced potent T cell proliferation effect and generated s trong CTL reaction against allogenic H22 cells. CONCLUSION: Lymphotactin genetic modification enhanced the in vitro immune activity of dendritoma.
