Role of plk1 in the anti-cancer effect of colcemid and vincri stine against K562 cells
- VernacularTitle:用共聚焦显微镜研究plk1在秋水仙胺和长春新碱抗肿瘤效应中的作用
- Author:
Wei HUANG
;
Yaozhen ZHANG
;
Jianfeng ZHOU
;
Wenli LIU
- Publication Type:Journal Article
- Keywords:
K562 cells;
Colchicum;
Vincristine;
Polo-like kinase
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of plk1 in the ant i-cancer effect of colcemid and vincristine against K562 cells. METHODS: K562 cells were treated with colcemid and vincristine a nd antisense oligonucleotide of plk1, then expression of plk1 and ?-tubulin wer e investigated by Western blotting and confocal microscopy. RESULTS: Treatment of K562 cells with colcemid and vincristine i nfluenced the condensation of plk1 and assembly of ?-tubulin, though without ch ange of protein quantity. Treatment with antisense oligonucleotide of plk1 not o nly reduced the expression of plk1 without influence on protein quantity of ?-t ubulin detected by Western blotting, but also disturbed the formation of centros ome observed by confocal microscopy. CONCLUSION: The function of colcemid and vincristine destructing the spindle might be realized through the mechanism of restraining condensation of plk1 and assembly of ?-tubulin, which might be dependent on plk1. plk1 may be a potential target in anti-cancer therapy.
