Gene polymorphism of insulin receptor substrate-1 in Northern Chinese Han pedigrees with type 2 diabetes mellitus
- VernacularTitle:北方汉族2型糖尿病人胰岛素受体底物1基因多态性的研究
- Author:
Difei WANG
;
Zhongxia QIU
;
Jin ZHANG
;
Lijuan JIANG
;
Dongmei CAI
;
Qingli LIU
;
Mei DING
- Publication Type:Journal Article
- Keywords:
Receptor, insulin;
Genes;
Diabetes mellitu s, non-insulin-dependent;
Insulin resistance
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of gene poly mo rphisms of insulin receptor substrate-1 (IRS-1) in northern Chinese Han pedigree s with type 2 diabetes mellitus (DM). METHODS: Polymorphisms in codon 804 and 971 of IRS-1 gene in 80 unrelated patients with type 2 DM and 80 control subjects were analyzed by polym erase chain reaction-sequence specific primer(PCR-SSP) technique followed by pol yacrylamide gel electrophoresis and silver staining. RESULTS: The frequencies (GCA→GCG) in codon 804 of IRS-1 gene w ere significantly higher in type 2 DM than normal subjects(0.200 vs 0.062, P
