Preparation and Content Determination of Collagen Protein Bilayer Minipellet of Recombinant Human Erythropoietin
- VernacularTitle:重组人红细胞生成素胶原蛋白双层微条的制备及含量测定
- Author:
Jiandong REN
;
Songqing LIU
;
Qing DAI
;
Yi HUANG
- Publication Type:Journal Article
- Keywords:
Recombinant human erythropoietin;
Collagen protein;
Bilayer minipellet;
Content determination
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare collagen bilayer minipellet of recombinant human erythropoietin(rhEPO)and for which to establish a content determination method.METHODS:The bilayer minipellet was prepared and shaped by pressuriz-ing gel with the collagen protein as coating material and the sodium chondroitin sulfate as releasing moderator;the content of rhEPO in minipellet was determined by RP-HPLC.RESULTS:The linear range of rhEPO was0.25～20?g/ml(r=0.9998),the average recovery rate was96.2%,the intraday RSD was0.9%and the interday RSD was2.6%(n=3).CONCLUSION:The preparation for the rhEPO bilayer minipellet is easy and the content determination method of which is accurate and reli-able.
