Simultaneous Determination of Blood Drug Concentration of Acetaminophen and Phenacetin by HPLC
- VernacularTitle:高效液相色谱法同时测定对乙酰氨基酚和非那西丁的血药浓度
- Author:
Hong ZHANG
;
Yu FANG
;
Shujun WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Acetaminophen;
Phenacetin;
Blood drug concentration
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the simultaneous determination of blood drug concentration of acetaminophen and phenacetin.METHODS:Diamonil C 18 was used as the chromatographic column,the mobile phase was methanol-water(45∶55),theophyllinum was used as internal standard,the detection wavelength was254nm and the column temperature was set at the room temperature.RESULTS:The minimum determination concentration of acetaminophen and phenacetin was5?g/ml,the calibration curve was linear in a range from5～25?g/ml;The recovery rate of both acetaminophen and phenacetin in this method was more than95%;both the intraday RSD and the interday RSD were less than4.0%.CONCLUSION:The method is rapid and accurate,which can satisfy the needs of the clinic blood drug concentration deter-mination of paracetamol and phenacetin.
