The Construction of bFGF/Math1 Gene Vector and Its Expression in the Cochlea of Rat
- VernacularTitle:bFGF/Math1基因表达载体的构建及在大鼠耳蜗中的表达
- Author:
Wei GUO
;
Shiming YANG
;
Suoqing ZHAI
- Publication Type:Journal Article
- Keywords:
Gene expression;
Lipofectin;
Basic fibr oblast growth factor(bFGF);
Math1
- From:
Journal of Audiology and Speech Pathology
1998;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the bFGF/Math1 f usion gene expression vector and to investigate its expression in the cochlea of rat. Methods Using the recombinant DNA technique to construct the bFGF/Math1 gene expression vector and the restriction enzyme analysis to ide ntify the correct construction. The mRNA expression was detected by the RT-PCR m ethod after being transfected into the cochlea of rat using lipofectin reagent. Results The recombinant was correct and the bFGF/Math1 wa s expressed in the cochlea of rat.Conclusion The PRK5-bFGF-Math1 eukaryotic expression plas mid was successfully constructed and the bFGF/Math1 was expressed in the mammali an cochlea.
