Compare with the three methods of DNA extraction of sweat latent fingerprint on the adhesive side of tapes
- VernacularTitle:3种提取胶带粘面汗潜指印中DNA的方法比较
- Author:
Deming WANG
;
Lihua GU
;
Jianjun YAN
- Publication Type:Journal Article
- Keywords:
Forensic physical evidence;
Adhesive tape;
Sweat latent fingerprint;
DNA genotype;
STR genotype
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To comparatively analyze the DNA extracted from sweat latent fingerprints on the adhesive side of tapes by three kinds of methods. Methods DNA was extracted from sweat latent fingerprint on the adhesive side of tapes using silicon bead test,QIA micro Kit and combined silicon bead-QIA micro Kit. STR loci were detected by multiplex PCR procedures. The PCR product was electrophoresed on an ABI 3100 Genetic Analyzer. Results 36%of the sweat latent fingerprints on the adhesive side of tapes was successfully genotyped using combined silicon bead-QIA micro Kit, and 21% using QIA micro Kit. Conclusion DNA genotyping of the sweat latent fingerprints on the adhesive side of tapes reveals higher possibility using combined silicon bead-QIA micro Kit than using QIA micro Kit and is less time-consuming.
